Rabbit Anti-RANKL (Receptor Activator of Nuclear Factor kappa B Ligand, Receptor Activator of NFkB Ligand, CD254, hRANKL2, hRANKL-2, Osteoclast Differentiation Factor, ODF, Osteoprotegerin Ligand, OPGL, sOdf, SOFA, TNF-related Activation-induced Cytokine, TRANCE, Tumor Necrosis Factor Ligand Superfamily Member 11, Tumor Necrosis Factor Superfamily Member 11, TNFSF11) (PE)
CD254 Antigen
CD254 is highly expressed by T lymphocytes and osteoblastic cells and plays a key role in the differentiation and activation of osteoclast precursors, acting as a ligand for both receptor activator of NF-KBeta (RANK) and the decoy receptor osteoprotegerin (OPG), which act as antagonists. Binding of CD254 to RANK is essential for the activation of osteoclastogenesis, stimulated by factors such as TNF-alpha, IL-1beta, IL-6 and IL-17, whereas binding to OPG neutralizes CD254, thereby inhibiting osteoclastogenesis.
Studies have shown that CD254 augments the dendritic cell (DC) stimulation of naïve T cell proliferation and may be involved in the regulation of DC-T cell interactions. Studies have also revealed a link between CD254 and apoptosis, through activation of the antiapoptotic kinase AKT/PKB and the mTOR/S6K signalling pathway.
Applications
Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.1-0.2ug/ml FLISA: 0.5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C for short-term only.Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant human CD254.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human CD254.
