Technical Data

R1170-02A
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
DB E
Crossreactivity
Hu
Accession #
NP_065812
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Raptor, phosphorylated (Ser863) (Regulatory-associated Protein of mTOR, Raptor, P150 Target of Rapamycin (TOR)-scaffold Protein, RPTOR, KIAA1303)

Raptor participates in the FRAP1 pathway and associates in a near stoichiometric ratio with FRAP1 to form a nutrient-sensitive complex (NSC). It plays a pivotal role as a scaffold protein in the FRAP1-signaling pathway and this interaction is essential for the catalyzed phosphorylation of EIF4EBP1. It has a positive role in nutrient-stimulated signaling to the downstream effector RPS6KB1. Under nutrient-deprived conditions, raptor serves as a negative regulator of FRAP1 kinase activity. Regulation of the interaction with FRAP1 is a critical mechanism by which cells coordinate the rate of cell growth and maintenance of cell size with different environmental conditions.

Applications
Suitable for use in ELISA and Dot Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Dot Blot: 1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser863 of human Raptor (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human Raptor when phosphorylated at Ser863.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Wang,Y., Circ. Res. 101(6), 560-569 (2007). 2.Fuchs,B.C., Am. J. Physiol., Cell Physiol. 293(1), C55-C63 (2007). 3.Olsen,J.V., Cell 127(3), 635-648 (2006). 4.Megan Cully, et al. Mol. Cell. Biol., Jan 2010; 30: 481-495. (E-pub)
USBio References
No references available
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