Technical Data

R1198-10G
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Isotype
IgG
Clone Number
C33A3
Grade
Purified
Applications
WB
Crossreactivity
Hu Mo
Gene ID
25780
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-RasGRP3

Lymphocyte activation occurs in part through activation of the Ras signaling pathway following lymphocyte receptor stimulation. The RasGRP family of guanine nucleotide exchange factors (GEFs) catalyzes the exchange of GDP for GTP on Ras family small GTPases, promoting their active GTP-bound form. Diacylglycerol (DAG) or phorbol ester binding to RasGRP family members causes their translocation to the cell membrane and stimulates their activity (1,2). While T-Cells express RasGRP1, B-cells express both RasGRP1 and RasGRP3. RasGRP3 is important in linking B-cell receptor (BCR) activation to Ras signaling (3). In response to BCR stimulation, RasGRP3 is phosphorylated at Thr133 by PKC. This phosphorylation event further activates RasGRP3 in response to DAG, which stimulates PKC activity (4,5).

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues near the C-teminus of human RasGRP3. Species sequence homology: monkey (100%)
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified
Specificity
Recognizes endogenous levels of total human RasGRP3. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
(1) Braun, D.C. et al. (2005) Mol Cancer Ther 4, 141–50. |(2) Lorenzo, P.S. et al. (2001) Cancer Res 61, 943–9. |(3) Oh-hora, M. et al. (2003) J Exp Med 198, 1841–51. |(4) Aiba, Y. et al. (2004) Proc Natl Acad Sci USA 101, 16612–7. |(5) Zheng, Y. et al. (2005) Blood 105, 3648–54.
USBio References
No references available
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