Rabbit Anti-Red Fluorescent Protein (RFP)
Well-characterizated antibodies against short-sequence epitope tags are common in the study of protein expression in systems.RFP-tags are composed of 15-residue peptides, VNGHEFEIEGEGEGR, derived from amino acids 22-36 of red fluorescent protein (RFP) from the Discosoma sea anemone.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: 0.05-0.2ug/ml Western Blot: 0.1-1.0ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Peptide corresponding to aa22-36, VNGHEFEIEGEGEGR from the Discosoma sea anemone, conjugated to KLH
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitue with 80ul sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes fusion proteins containing RFP-tags and RFP variants, such as mCherry, tdTomato, DsRed, DsRed2, mOrange. Does not react with GFP or GFP-tagged proteins.
