Technical Data

R1425-21F
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-Relaxin 3 (Relaxin-3, RLN3, RXN3, H3, Insulin-like Peptide 7, Insulin-like Peptide INSL7, INSL7, Prorelaxin H3, UNQ6188/PRO20213, ZINS4)

Human Relaxin 3 (H3 relaxin, INSL7) is one of seven relaxin-like peptides belonging to the insulin superfamily. Unlike human relaxins 1 and 2, it does not play a role in reproduction but appears to be a neuropeptide involved in stress response in the brain stem. The 142aa Relaxin 3 pre-proprotein is processed to remove a 25aa signal peptide and a connecting peptide (aa53-118). The resulting mature Relaxin 3 is a 5.5kD, 51aa secreted heterodimer of A (aa119-142) and B (aa26-52) peptides connected by two intermolecular disulfide bonds. Relaxin 3 is the only known ligand for the G-protein-coupled receptor RXFP3. In rodents, GPCR135 is expressed primarily in the supraoptic and paraventricular nucleus. This region has connections to the dorsal tegmental region of the pons (also called the nucleus incertus), where expression of Relaxin 3 is highest. Relaxin 3 also binds the more widely expressed LGR7 (RXFP1) receptor, but with lower affinity than that of Relaxin 2. Although binding of Relaxin 3 to LGR7 increases intracellular cAMP, binding to GPCR135 inhibits cAMP accumulation, indicating coupling to Gi, Go or Gz by this receptor. Relaxin 3 expression does not overlap well with its other receptor, GPCR142, which instead appears to be the primary receptor for INSL5.

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.1ug/ml Immunohistochemistry (Fixed frozen): 5-15ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant corresponding to aa27-142 from human Relaxin 3 expressed in E.coli.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Relaxin 3. Species Crossreactivity: Recombinant human mature Relaxin 3 (<1%).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Kizawa, H. et al. (2003) Regul. Pept. 113:79. 2. Wilkinson, T.N. et al. (2005) BMC Evol. Biol. 5:14. 3. Bathgate, R.A. et al. (2006) Pharmacol. Rev. 58:7. 4.Liu, C. et al. (2005) Ann. N.Y. Acad. Sci. 1041:47. 5. Tanaka, M. et al. (2005) Eur. J. Neurosci. 21:1659. 6. Liu, C. et al. (2003) J. Biol. Chem. 278:50754. 7. Rosengren, K.J. et al. (2006) J. Biol. Chem. 281:5845. 8. Liu, C. et al. (2003)J. Biol. Chem. 278:50765.
USBio References
No references available
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