Rabbit Anti-RENT1 (Regulator of Nonsense Transcripts 1, ATP-dependent Helicase RENT1, Delta Helicase, FLJ43809, FLJ46894, hUPF1, KIAA0221, Nonsense mRNA Reducing Factor 1, NORF1, pNORF1, pNORF-1, UP Frameshift 1, UPF1, Up Frameshift Mutation 1 Homolog (S. cerevisiae), Up-frameshift Mutation 1 Homolog (S. cerevisiae), Up Frameshift Suppressor 1 Homolog, UPF1 Regulator of Nonsense Transcripts Homolog, Yeast Upf1p Homolog) (MaxLight 550)
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
RENT1 is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. RENT1 is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Positive Control
U2OS cell lysate
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic linear peptide corresponding to RENT1. Species sequence homology: mouse, rat, bovine and canine.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human RENT1.
