Rabbit Anti-Resistin-like Molecule, beta (RELMb) (Not for Export EU)
Resistance to insulin characterizes type 2 diabetes, the most common form of diabetes. There is a strong link between type 2 diabetes and obesity, as most patients tend to be obese. Thiozolidinediones (TZDs), a new class of anti-diabetic drugs, enhances target-tissue sensitivity to insulin. A screen of genes down regulated by TZD in adipocyte led to the discovery of a new protein hormone called resistin (for resistance to insulin). Resistin, specifically produced and secreted by adipocyte, is present at elevated levels in the blood of obese animals, and is down regulated by fasting and anti-diabetic drugs. Antibody to resistin stimulated glucose uptake and improved insulin sensitivity in obese mice. Some other protein related to resistin has been called resistin-related molecules (RELM-alpha and beta). Resistin family of proteins was also identified as proteins (FIZZ1-3, for Found in Inflammatory zone) involved in allergy and inflammation.
RELM-beta/FIZZ2 (prepeptides: mouse 105 aa; human 11 aa ~45-65% identity with FIZZ1 and 2) is highly related to resistin at the C-terminus region. Its expression is most abundant in colon (proximal and distal colon) but not in white adipose tissue. In mouse, RELM-beta is also detected at low levels in cecum and ileum. In min mouse (mouse model for human familial adenomatosus polyposis), RELM-beta expression was markedly increased in tumors immediately adjacent to normal tissue.
A 14-aa peptide sequence near the N-terminus of Mouse RELM-beta (1) was coupled with KLH (carrier protein) and antibodies generated in rabbits. Antibodies have been affinity purified over the control-peptide Sepharose.
Applications
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended Dilution
Western Blotting 1-5ug/ml for affinity pure using Chemiluminescence technique. Resistin is ~9kD.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A 14-aa peptide sequence near the N-terminus of Mouse RELM-beta (1) was coupled with KLH (carrier protein)
Form
Supplied as a liquid, neat serum, 40% glycerol.
Purity
Purified over the control-peptide Sepharose
Specificity
Mouse sequence is only 42% conserved in human RELM-beta. We recommend the use of antibody that is raised to human RELM-beta sequence. No significant sequence was found with resistin or RELM-alpha. Antibody crossreactivity in various species is not established. We recommend the use of control peptide (use 5-10 ug of peptide per 1 ug of aff pure or 1 ul of antiserum) to demonstrate antibody specificity.
