Rabbit Anti-Ribosomal Protein S3
Ribosomal protein S3 (rpS3) is a component of the 40S ribosomal subunit and is involved in translation. HSP90 interacts with both the N-terminus and C-terminus of rpS3, preventing its ubiquitination and degradation and thereby retaining the integrity of the ribosome (1). rpS3 has also been shown to function as an endonuclease during DNA damage repair (2,3). Furthermore, overexpression of rpS3 sensitizes lymphocytic cells to cytokine-induced apoptosis, indicating a third role for rpS3 during apoptosis (4). The functions of rpS3 during DNA damage repair and apoptosis have been mapped to two distinct domains (4).
Applications
Suitable for use in Immunofluorescence/Immunocytochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunofluorescence/Immunocytochemistry: 1:25 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide derived from the sequence of human ribosomal protein S3.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes endogenous levels of human total ribosomal protein S3 at 27kD. Species Crossreactivity: mouse, rat and monkey
