Rabbit Anti-RICK, NT (RIP2, CARDIAK, RIP-like Interacting CLARP Kinase, Receptor Interacting Protein-2, CARD-containing ICE Associated Kinase)
Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase was recently identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively. RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain.
Overexpression of RICK induced apoptosis and activation of NF-kB and JNK. RICK interacts with members of the TRAF family, CLARP and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of proinflammatory cytokine IL-1b. The messenger RNA of RICK is expressed in multiple human tissues.
Applications
Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunofluorescence. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1ug/ml Immunocytochemistry: 10ug/ml. Immunofluorescence: 20ug/ml. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 20 amino acids near the N-terminus of human RICK. Species sequence homology: bovine (95%)
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human RICK. Species Crossreactivity: mouse and rat
