Rabbit Anti-RIG-1 (Retinoic Acid Inducible Gene 1 Protein, RIG-I, Probable ATP-Dependent RNA Helicase DDX58, Dead Box Protein 58, DDX58) (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
RIG-I is a receptor found in the cytoplasm that recognizes viral RNA from replicating viruses in infected cells. Upon activation with intracellular RNA, a cascade is triggered resulting in the activation of NF-kappa-B, and the eventual induction of antiviral cytokines such as IFN-beta and RANTES. RIG-I is essential for the production of interferons in response to RNA viruses and as such play a key role in innate immunity.
Applications
Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate, pH 6, is recommended.Optimal dilutions to be determined by the researcher.
Positive Control: Human heart
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Human GST-tagged RIG-1 protein.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by DEAE chromatography.
Specificity
Recognizes human RIG-I. Species Crossreactivity: mouse and rat
