Mouse Anti-rSec6
rSec6 is an 87kD rat homolog of yeast Sec6p, a protein involved in protein transport between the Golgi apparatus and the plasma membrane. In yeast, Sec6p is part of a high molecular weight complex which includes Sec8p and Sec15p, that associates with the ras-like GTP binding protein Sec4p. An analogous 17S complex in rat brain was found to contain eight novel proteins, including rSec6 and rSec8, with a combined molecular weight of 734kD. Membrane-associated rSec6/8 complex has been shown to co-immunoprecipitate with syntaxin, and is ubiquitously expressed, further evidence that this complex is involved in the mammalian exocyst complex.
Applications
Suitable for use in Immunohistochemistry (paraffin sections), Immunocytochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot (ECL): 1:250. Optimal dilutions to be determined by researcher.
Positive Controls
Rat Brain Tissue Extract. Mouse Brain Tissue Extract
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to rat rSec6.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes rat Sec6 at 87kD. Species Crossreactivity: human, mouse, rat, bovine, canine, chicken, hamster, sheep, porcine, monkey and rabbit. Immunofluorescence of PC12 cells shows co-localization of rSec6 with vesicles and sites of exocytosis.