Rabbit Anti-RSK1 (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ribosomal Protein S6 Kinase alpha-1, RPS6KA1, S6K-alpha-1)
Ribosomal Protein S6 Kinase 90kDa Polypeptide 1, EC=2.7.11.1, HU-1
Rsk1, Rsk2, and Rsk3 (ribosomal protein S6 kinase, 90kDa, polypeptide 1,2,3; S6K-alpha 1,2,3) are Ser/Thr kinases which are activated by MAP Kinases, and are alternatively referred to as MAPKAP kinases 1a, 1b, and 1c. Following activation, Rsk isoforms translocate to the nucleus, where Rsk2 phosphorylates Ser10 of Histone H3, a critical step in the stimulation of immediate-early gene expression. Rsk1 is able to phosphorylate Ser133 of CREB, activating it as a transcription factor. Rsk1 activation can be monitored by phosphorylation state specific antibodies directed against sites involved in its activation.
Applications
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:1000 detected RSK1 on 10ug of NIH/3T3 lysates. Immunoprecipitation: 4ug was used per 1500ug of crude protein sample Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to the CT sequence of human p90 RSK1 (C-LAQRRVRKLPSTTL).
Form
Supplied as liquid in TBS, 0.01% Sodium Azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human p90 RSK1. Species Crossreactivity: mouse and rat
