Mouse Anti-Roundabout Homolog 1 (H-Robo-1, Deleted in U twenty twenty, ROBO1, DUTT1)
Roundabout homolog 1 belongs to the neural adhesion molecule (NCAM) family of receptors. It is a receptor for SLIT1 and SLIT2 which are thought to act as a molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. It may be required for lung development. There are three named isoforms. It is widely expressed, with the exception of kidney. Defects in Roundabout homolog 1 may be a cause of breast and lung cancer.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20°C. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Partial recombinant human Roundabout homolog 1 (aa491-589) with a GST tag.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human Roundabout homolog 1.
