Technical Data

R9931-16A
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E WB
Crossreactivity
Hu Mo
Accession #
NP_006657
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Reptin, CT (RuvB-like 2, 48kD TATA Box-binding Protein-interacting Protein, 48kD TBP-Interacting Protein, TIP49b, Repressing Pontin 52, Reptin 52, 51kD Erythrocyte Cytosolic Protein, ECP-51, TIP60-associated Protein 54-beta, TAP54-beta, TIP48, TIP49B, RUVBL2, INO80J, CGI-46)

This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 400-430 in the C-terminal region of human Reptin. Species Crossreactivity: bovine and xenopus.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human Reptin. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Bauer, A., et al., EMBO J. 19(22):6121-6130 (2000). 2.Parfait, B., et al., Ann. Genet. 43(2):69-74 (2000). 3.Wood, M.A., et al., Mol. Cell 5(2):321-330 (2000). 4.Salzer, U., et al., Biochim. Biophys. Acta 1446(3):365-370 (1999). 5.Kanemaki, M., et al., J. Biol. Chem. 274(32):22437-22444 (1999).
USBio References
No references available
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