Technical Data

S0095-30
Clone Type
Monoclonal
Host
Mouse
Source
Rat
Isotype
IgG1
Clone Number
4E202
Grade
Affinity Purified
Applications
IC IHC IP WB
Crossreactivity
Ca Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-SAP97 (Synapse-associated Protein 97, Discs Large Homolog 1, DLG1, dJ1061C18.1.1, DKFZp761P0818, DLGH1, HDLG, Presynaptic Protein SAP97)

Synapse-associated proteins SAP97 and SAP102 display extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminals. In addition to the CNS, SAP97 is also detected at the basal lateral membrane between a variety of epithelial cells. SAP102 associates with NMDA receptors through the C-terminal NR2B subunit of the receptor. The N-terminal SAP97 PDZ domain mediates its interaction with the synaptic ras-GTPase activating protein SynGAP.

Applications
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Electron Microscopy. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 (ECL). Detects bands at ~97kD and 140kD. Optimal dilutions to be determined by researcher.
Positive Controls
Rat Brain Tissue Extract, Mouse Brain Tissue Extract
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to rat SAP97 (N-Terminal fragment).
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes rat SAP97. Species Crossreactivity: human, mouse and canine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Muller, B.M., Kistner, U., Veh, R.W., Cases-Langhoff, C., Becker, B., Gundelfinger, E.D., Garner, C.C. (1995) J. Neurosci. 15: 2354-2366. 2. Reuver, S., Garner, C. (1998) J. Cell Sci. 111: 1071-1080. 3. Kim, E., and Sheng, M. (1996) Neuropharmacolog 35: 993-1000. 4. Thomas, U., Phannavong, B., Muller, B., Garner, C.C., Gundelfinger, E.D. (1997) Mech Dev. 62: 161-174. 5. Suzuki, T., Ito, J., Takagi, H., Saitoh, F., Nawa, H., Shimizu, H. (2001) Brain Res Mol Brain Res 89(1-2): 20-28. 6. Mehta, S., Wu, H., Garner, C.C., Marshall, J., (2001) J Biol Chem 276(19): 16092-16099. |
USBio References
No references available
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