Technical Data

S0097-71
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2b
Clone Number
6D754 (Sarmy-1)
Grade
Purified
Applications
IP
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-SARM (SAM and ARM-containing Protein)

In mammals, TIR domain adapters, including Myd88, TIRAP, TICAM-1 (also called TRIF), and TRAM, act in Toll-like receptor (TLR) signaling pathway. The last member of this family called SARM (sterile and armadillo motifs) is highly conserved but has no known function in any organism. SARM is the only mammalian TIR-domain adapter proteins conserved in C. elegans, suggesting that SARM is the most ancient member of this class of signaling proteins. Overexpression of SARM fails to activate either NF-kB- or IRF-3-dependent reporter gene expression, two transcription factors that are sensitive to TLR signaling, suggesting that SARM is functionally distinct from other mammalian TIR domain-containing proteins.

Applications
Suitable for use in Immunoprecipitation. Not suitable for Western Blot. Other applications not tested.
Recommended Dilutions
Immunoprecipitation: 1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to aa93-292 from human SARM.
Form
Supplied as a liquid in PBS, 0.02% sodium azide, 10% glycerol.
Purity
Purified from concentrated hybridoma tissue culture supernatant.
Specificity
Recognizes human SARM.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
11. Requirement for a conserved Toll/interleukin-1 resistance domain protein in the Caenorhabditis elegans immune response: N.T. Liberati, et al.; PNAS 101, 6593 (2004) 2. TLR-independent control of innate immunity in Caenorhabditis elegans by the TIR domain adaptor protein TIR-1, an ortholog of human SARM: C. Couillault, et al.; Nat. Immunol. 5, 488 (2004)
USBio References
No references available
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