S0099-010
Clone Type
PolyclonalHost
RabbitSource
MouseIsotype
IgGGrade
SerumApplications
E IC IF IHC WBCrossreactivity
Bo Hm Mo RtShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Scavenger Receptor BII (SR-BII) (Not for Export EU)
High density lipoproteins (HDLs) play a critical role in cholesterol metabolism. HDL plasma concentrations are inversely correlated with risk for atherosclerosis. SR-BI and SR-BII (previously known as SR-BI.2) are the alternatively spliced products of a single gene. SR-BII differs from SR-BI in that the encoded c-terminal cytoplasmic domain is almost completely different. SR-BII binds HDLs mediating selective uptake of HDL cholesteryl ester; with an approximately 4-fold lower efficiency than SR-BI. Nuclease protection assays show SR-BII to be abundant in mouse tissues expressing SR-BI. SR-BII expression found in liver, adrenal glands, and testes. The role of SR-BII is not completely clear. Research suggests that it may be a functional HDL receptor. SR-BII mRNA results from the alternative splicing of SR-BI precursor transcripts with the SR-BII isoform mediating selective transfer of lipid between HDL and cells. The relative expression and functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells (1-6).
Applications
Suitable for use in Immunohistochemistry, Immunocytochemistry, Western Blot and Immunofluorescence. Knockdown Validated. Other applications not tested
Recommended Dilution
Western Blot: 1:1000. A band of ~82kD is seen in tissues that express SR-BII such as liver, tested and adrenal glands. Immunofluorescence (IC): 1:25-1:100 Immunohistochemistry (paraffin): 1:25 Optimal dilution determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Peptide corresponding to residues from mouse SR-BII (between residues 400-478) plus an N-terminal cysteine coupled to KLH.
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity
Recognizes mouse SR-BII. Species crossreactivity: mink, rat, bovine and hamster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. N.R. Webb, et al. SR-BII, an Isoform of the Scavenger Receptor BI Containing an Alternate Cytoplasmic Tail, Mediates Lipid Transfer between High Density Lipoprotein and Cells. J. of Biol. Chem. 273(24): 15241-15248 1998. 2. N.R. Webb, et al. Alternative forms of the scavenger receptor B1 (SR-B1). J. Lipid Research 38: 1490-1495, 1997. 3. M. Krieger. The "best" of cholesterols the "worst" of cholesterols: A tale of two receptors. PNAS 95: 4077-4080, 1988. 4. S. Acton, et al. Identification of Scavenger Receptor SR-BI as a High Density Lipoprotein Receptor. Science 271: 518-520, 1996. 5. Rigotti, et al. A targeted mutation in the murine gene encoding the high density lipoprotein (HDL) receptor scavenger receptor class B type I reveals its key role in HDL metabolism. PNAS 94: 12610-12615, 1997. 6. R.E. Temel, et al. Scavenger receptor class B, type I (SR-BI) is the major route for the delivery of high density lipoprotein cholesterol to the steroidgenic pathway in cultured mouse adrenocortical cells. PNAS 94: 13600-13605, 1997.USBio References
No references available