Technical Data

S0099-15B
Clone Type
Polyclonal
Host
Sheep
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Sheep Anti-Scavenger Receptor, Cysteine Rich Domain (SRCRB4D, Group B (4 Domains) S4D-SRCRB, SRCRB-S4D)

SRCRB4D (Scavenger receptor cysteine-rich domain group B four B-type domain containing) is a 56 kD (predicted), secreted member of the SRCR superfamily. It is widely expressed and likely plays some role in innate immunity. Mature human SRCRB4D is 528aa in length. It contains four SRCR domains (aa58-573), each of which possesses three intradomain disulfide bonds. Although it contains no potential N-linked glycosylation sites, other types of posttranslational modification cannot be ruled out. There are three potential isoform variants. One shows a three aa substitution for aa 271-575, a second shows an 11aa substitution for aa 1-24, and a third shows a 25aa substitution for aa1-416, accompanied by a six aa insertion after Gly445. Over aa 45-575, human and mouse SRCRB4D share 90% aa identity.

Applications
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1.0ug/ml HepG2 human hepatocellular carinoma cell line. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Ala45-Ser575 from human SRCRB4D, expressed in CHO cells.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human SRCRB4D.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net