Rabbit Anti-Scavenger Receptor BI (SR-BI)
High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. The SR-BI binds HDLs and mediates selective uptake of HDL cholesteryl ester. SR-BI binds HDL with high affinity, is expressed primarily in liver and nonplacental steroidgenic tissues, and mediates selective cholesterol uptake by a distinct mechanism. In mice, it seems that SR-BI plays a key role in determining the levels of plasma lipoprotein cholesterol and the accumulation of cholesterol stores in the adrenal gland.
Applications
Suitable for use in Western Blot and Immunoprecipitation, Immunocytochemistry and Immunofluorescence.. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 KO Validated: Immunofluorescent analysis of SR-BI localization in aorta of Tie2-Scarb1 x Scarb1-KO and Scarb1-KO mice. Mouse aorta sections were stained for SR-BI (a and d, red). In several EC stained for SR-B1 red color was present on both apical and basolateral sides. There is no red signal in aorta from Scarb1-KO mice. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2015/607120/) licensed under a CC-BY license. Optimal dilutions to be determined by the researcher.
Positive Control
HL-60 cells
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
C-terminal peptide containing residues from mouse SR-BI (within residues 450-509). Species Sequence Homology: bovine
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse SR-BI (~82kD). Species Crossreactivity: rat and hamster SR-BI
