Goat Anti-SCAP2 (Src Family Associated Phosphoprotein 2, Src Kinase Associated Phosphoprotein 2, SKAP2, Fyn-associated Phosphoprotein SKAP55 Homolog, MGC10411, MGC33304, Pyk2/RAFTK-associated Protein, PRAP, Retinoic Acid-induced Protein 70, RA70, SAPS, SKAP-HOM, Src Associated Adaptor Protein, Src Kinase-associated Phosphoprotein of 55-related Protein, SKAP55R)
src-associated adaptor protein, src kinase-associated phosphoprotein of 55-related protein, src family associated phosphoprotein 2, Pyk2/RAFTK-associated protein, Fyn-associated phosphoprotein SKAP55 homologue, SKAP55R, SKAP-HOM, SCAP2, SAPS, RA70, MGC33304, MGC10411, src family associated phosphoprotein 2, PRAP, SCAP2, SKAP2
The protein encoded by this gene belongs to the src family kinases. This protein is similar to the src kinase associated phosphoprotein 1. It is an adaptor protein that is thought to play an essential role in the src signaling pathway in various cells. It inhibits PTK2B/RAFTK activity and regulates alpha-synuclein phosphorylation.
Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Peptide ELISA Titer: 1:32,000 Western Blot: 0.5-2ug/ml ~38kD band observed in lysates of cell line Jurkat (calculated MW of 41.2kD according to NP_003921.2). In transfected HEK293 transiently expressing SKAP2 a band of approx. 45kD is observed. This band is not observed in the non-transfected HEK293. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-GLVPKAYIMEMYDI, from the C Terminus of human SCAP2 (NP_003921.2).
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes human SCAP2. Species sequence homology: mouse, rat, canine, bovine.
