Technical Data

S0668-55J1
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
PE
Isotype
IgG1
Clone Number
12K173
Grade
Affinity Purified
Applications
FC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Mouse Anti-Semaphorin 4D (BB18, A8, GR3, CD100 antigen, SEMA4D, CD100, SEMAJ) (PE)

Semaphorin 4D (Sema4D/CD100, previously sem J, G, or C-like 2) is a widely expressed 150kD type I transmembrane glycoprotein of the Class 4 Semaphorin family with activity in the immune and nervous systems. The 861aa Semaphorin 4D contains a 710aa extracellular domain (ECD) with a sema domain and an immunoglobulin-like domain. Within the ECD region used as an immunogen, mouse Semaphorin 4D shares 87% and 93% aa identity with human and rat Semaphorin 4D, respectively. A soluble, active 120kD form is produced proteolytically upon activation of B and T cells. Semaphorin 4D binds CD72 on B cells and dendritic cells and Plexin B1 elsewhere. In developing neurons, Semaphorin 4D assists in guidance. Semaphorin 4D is pro-angiogenic when expressed by tumor-associated macrophages.

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Absorption
488nm
Emission
565-605nm
Storage and Stability
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Immunogen
Recombinant corresponding to extracellular domain, Met22-Arg734, of human Semaphorin 4D expressed in CHO cell line.
Form
Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Semaphorin 4D.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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