Technical Data

S0820
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IP WB
Crossreactivity
Hu Mk
Gene ID
23513
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Scribble (Scribbled homolog (Drosophila), CRIB1, hScrib, KIAA0147, LAP4, Protein LAP4, Protein scribble homolog, SCRB1, SCRIB1, Vartul, VARTUL)

Scribble (Scrib) was originally identified in a genetic screen in Drosophila along with cell polarity determinants Discs Large (Dlg) and Lethal giant larvae (Lgl). Drosophila mutants homozygous for these genes share similar phenotypes, including the loss of apicobasal cell polarity and neoplastic tissue overgrowth. These phenotypic similarities suggest that these three proteins function in a common pathway important for establishing and maintaining apicobasal polarity in epithelial cells (1,2). Scribble contains many leucine-rich repeats and PDZ domains important for localizing scribble to adherens junctions and basolateral regions of mammalian epithelial cells (3). Scribble reportedly binds beta-catenin, APC, E-cadherin and the E6 protein from high-risk virus type of HPV through a short motif important for E6-induced cell transformation (4-8). Overexpression of scribble inhibits transformation of rodent epithelial cells by HPV E6/7 proteins (8).

Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to amino acid residues surrounding Gly1237 of human scribble protein.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes endogenous levels of total human Scribble. Species crossreactivity: monkey.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Bilder, D. and Perrimon, N. (2000) Nature 403, 676-80, 2. Bilder, D. et al. (2000) Science 289, 113-6, 3. Humbert, P.O. et al. (2008) Oncogene 27, 6888-907, 4. Sun, Y. et al. (2009) Mol Biol Cell 20, 3390-400, 5. Qin, Y. et al. (2005) J Cell Biol 171, 1061-71, 6. Navarro, C. et al. (2005) Oncogene 24, 4330-9, 7. Takizawa, S. et al. (2006) Genes Cells 11, 453-64, 8. Nguyen, M.L. et al. (2003) J Virol 77, 6957-64.
USBio References
No references available
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