S0950-10D
Clone Type
PolyclonalHost
GoatSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
Bo Ca Hu MoAccession #
NP_004565.1, NP_536341.1, NP_001185642.1Gene ID
5414, 18952Shipping Temp
Blue IceStorage Temp
-20°CGoat Anti-SEPT4 (Septin-4, Peanut-like Protein 2, Brain Protein H5, Cell Division Control-related Protein 2, hCDCREL-2, Bradeion beta, CE5B3 beta, Cerebral Protein 7, hucep-7, SEP4, PNUTL2)
PNUTL2 is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. The protein is thought to be part of a complex involved in cytokinesis.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:64,000 Western Blot: 1-3ug/ml, human and mouse heart lysates observed on ~50kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-RNKLTRESGTD, from human SEPT4, at the internal region of the protein (NP_004565.1, NP_536341.1, NP_001185642.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SEPT4. Species Crossreactivity: mouse. Species sequence homology: Bovine and canine.