Technical Data

S1000-89D
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
FC WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-SERPINB9 (Serpin B9, Cytoplasmic Antiproteinase 3, CAP-3, CAP3, Peptidase Inhibitor 9, PI-9, PI9)

Mouse Spi-6, also known as serpin B9, is a cytoplasmic serine protease inhibitor of ~42kD. Spi-6 is expressed in dendritic cells, lymphocytes and specific cell types at immune- privileged sites. It inactivates granzyme B, a natural occurring inducer of programmed cell death. As such, Spi-6 functions as anti-apoptotic protein. In cytotxic T cells (CTL), Spi-6 is required to protect clonal bursts (expansion) from granzyme B induced cell death. However, it is not required for the development of memory cells. Expression of SPI-6 is of crucial importance in the escape of several tumors from a CTL response and is, therefore, a parameter that influences the feasibility of CTL-mediated immunotherapy of cancer.

Applications
Suitable for use in Western Blot and Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:50 Western Blot: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant corresponding to Spi-6 conjugated to GST.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes mouse Spi-6.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Zhang, M et al; Differential survival of cytotoxic T cells and memory cell precursors. J Immunol 2007, 178: 3483. 2. Zhang, M et al; Serine protease inhibitor 6-deficient mice have increased neutrophil immunity to Pseudomonas aeruginosa. J Immunol 2007, 179: 4390.
USBio References
No references available
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