Technical Data

S1012-10-ML650
Clone Type
Monoclonal
Host
Mouse
Source
Rat
Conjugate
MaxLight™650
Isotype
IgG1,k
Clone Number
5H43 (84)
Grade
Affinity Purified
Applications
IC IP WB
Crossreactivity
Hu Mo Rt
Accession #
NM_031892
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Mouse Anti-SETA (CIN85, Ruk, SH3KBPI) (MaxLight 650)

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

SETA (CIN85, Ruk/SH3KBPI) regulates the ligand-dependent endocytosis of the IgE receptor.
Applications
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml detects SETA in RIPA lysates from NG108-15 cells. Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate SETA in human U87 glioma cells and rat primary astrocytes. Immunocytochemistry: Has been reported by an independent laboratory to detect SETA in rat primary astrocytes. Optimal dilutions to be determined by the researcher.
Recommended secondary antibodies
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
6His and HA tagged fusion protein corresponding to full length rat SETA.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes rat SETA, Mr ~85kD. SETA has multiple splice variants which can result in multiple bands in Western Blot. Species Crossreactivity: human and mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Finniss, S., et al., Biochem. Biophys. Res. Comm. 325: 174-182, 2004. 2. Chen, B., et al., J. Biol. Chem. 275: 19275-19281, 2000. 3. Bogler, O., et al., Neuro-Oncology 2: 6-15, 2000.
USBio References
No references available
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