Technical Data

S1012-86G
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC
Crossreactivity
Bo Hu Mo
Accession #
NP_003003.3
Gene ID
6422
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-SFRP1 (Secreted Apoptosis-related Protein 2, SARP-2, Secreted Frizzled-related Protein 1, sFRP-1, FRP-1, SARP2, FRP1, FRP)

SFRP1 encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer.

Applications
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1000 Immunohistochemistry (Paraffin): 3-6ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to KNHECPTFQSVFK, from human SFRP1, at C-terminal (NP_003003.3).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SFRP1. Species sequence homology: Bovine and mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Gumz ML, Zou H, Kreinest PA, Childs AC, Belmonte LS, LeGrand SN, Wu KJ, Luxon BA, Sinha M, Parker AS, Sun LZ, Ahlquist DA, Wood CG, Copland JA Secreted frizzled-related protein 1 loss contributes to tumor phenotype of clear cell renal cell carcinoma Clin Cancer Res. 2007 Aug 15;13(16):4740-9
USBio References
No references available
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