S1013-29H
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
IP WBCrossreactivity
Hu Mo RtGene ID
5781Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-SHP 2, phosphorylated (Tyr580) (SHPTP-2, PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp, PTPN11)
SHP-2 (PTPN11) is a ubiquitously expressed, nonreceptor protein tyrosine phosphatase (PTP). It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens, and extracellular matrices in the control of cell growth, differentiation, migration, and death (1). Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines (2). In addition to its role in Gab1-mediated Erk activation, SHP-2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 p85 binding sites (3). SHP-2 becomes phosphorylated at Tyr542 and Tyr580 in its carboxy-terminus in response to growth factor receptor activation (4). These phosphorylation events are thought to relieve basal inhibition and stimulate SHP-2 tyrosine phosphatase activity (5). Mutations in the corresponding gene result in a pair of clinically similar disorders (Noonan syndrome and LEOPARD syndrome) that may result from abnormal MAPK regulation (6).
Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Tyr580 of human SHP-2. Species Sequence Homology: Chicken.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes endogenous levels of human SHP 2 at ~72kD only when phosphorylated at Tyr580. Species Crossreactivity: Mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Qu, C.K., Cell Res. 10: 279-288 (2000). 2. Maroun, C.R., et al., Mol. Cell Biol. 20: 8513-8525 (2000). 3. Zhang, S.Q., et al., Mol. Cell Biol. 22: 4062-4072 (2002). 4. Bennett, A.M., et al., Proc. Natl. Acad. Sci. USA 91: 7335-7339 (1994). 5. Lu, W., et al., Mol. Cell 8: 759-769 (2001). 6. Edouard, T., et al., Cell Mol. Life Sci. 64: 1585-1590 (2007).USBio References
No references available