Rabbit Anti-SHPRH (SNF2 Histone-linker PHD RING-finger Helicase, 2610103K11RIK, bA545I5.2, FLJ27258, FLJ37625, FLJ45012, FLJ90837, KIAA2023, MGC134886)
SHPRH is a member of the SWI/SNF family of ATPaes/helicases, it posesses a C3HC4 RING motif characteristic of ubiquitin ligase protein and it has a fundamental role in DNA repair mechanism. Upon genotoxic stress, SHPRH associates with PCNA, RAD18, and the ubiquitin-conjugating enzyme UBC(E2) and promotes methyl methanesulfonate (MMS)-induced PCNA polyubiquitation suppressing genomic instability. SHPRH gene is mutated in a number of cancer cell lines, including those from melanomas and ovarian cancer.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:500 Western Blot: 1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide (DKTKKQAVGSPRKIQKETRK) corresponding to aa 521-540 of human SHPRH
Form
Supplied as a liquid in PBS, pH 7.2.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human SHPRH
