Technical Data

S1013-34F
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Serum
Applications
E IHC RIA WB
Crossreactivity
Hu Rt
Gene ID
3381
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Rabbit Anti-Sialoprotein, Bone (BSP) (Not for Export EU)
BSP, Bone sialoprotein 2, BSP2, Cell-binding sialoprotein, Integrin-binding sialoprotein

Bone sialoprotein (BSP) is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70-80kD. The protein has been found in osteoblasts and osteocytes. BSP is synthesized and secreted by bone-, dentin- and cementum-forming cells. Diseases concerning the bone turnover, pathological bone alterations as well as the rapid increase of osteoporosis make it necessary to establish new markers. Bone sialoprotein is discussed as a potential serum marker for monitoring bone remodelling.

Applications
Suitable for use in ELISA, RIA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (paraffin): 1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Human BSP (bone extract)
Form
Supplied as a lyophilized powder. No preservative added. Reconstitute with 20ul/100ul of sterile dH2O.
Purity
Serum
Specificity
Recognizes human bone sialoprotein. Does not crossreact with human osteopontin or osteonectin. Species Crossreactivity: rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Karmatschek M, Maier I, Seibel MJ, Woitge HW, Ziegler R, Armbruster FP. Improved purification of human bone sialoprotein and development of a homologous radioimmunoassay. Clin Chem 1997;|43:2076-82. 2. Bellahcene A, Van Riet, I, de Greef C, Antoine N, Young MF, Van Camp B, Castronovo V. Bone|sialoprotein mRNA and protein expression in human multiple myeloma cell lines and patients. Br J|Haematol 2000; 111:1118-21. 3. Adwan H, Bauerle T, Najajreh Y, Elazer V, Golomb G, Berger MR.:Decreased levels of osteopontin andbone sialoprotein II are correlated with reduced proliferation, colony formation, and migration of GFPMDA- MB-231 cells.Int J Oncol. 2004 May;24(5):1235-44.
USBio References
No references available
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