Technical Data

S1013-60N3
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
FITC
Isotype
IgG1
Clone Number
9A202
Grade
Affinity Purified
Applications
FC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Siglec 7 (Sialic Acid Binding Immunoglobulin-like Lectin 7, Sialic Acid-binding Ig-like Lectin 7, Siglec 7, Adhesion Inhibitory Receptor Molecule 1, AIRM1, AIRM-1, CD328 Antigen, CDw328, D-siglec, P75, QA79) (FITC)
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Hybridoma
P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Monocyte-derived dendritic cells.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human CDw328.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Falco, M. et al. (1999) Identification and molecular cloning of p75/AIRM1, a novel member of the sialoadhesin family that functions as an inhibitory receptor in human natural killer cells. J. Exp. Med. 190: 793-801, 2. Nicoll, G. et al. (1999) Identification and characterization of a novel Siglec, Siglec-7, expressed by human natural killer cells and monocytes*. J. Biol. Chem. 274: 34089-34095.
USBio References
No references available
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