Mouse Anti-Siglec 7 (Sialic Acid-binding Immunoglobulin-like Lectin, CDw328) (Biotin)
Siglec 7 is a type I transmembrane glycoprotein and member of the Siglec (sialic acid binding Ig-like lectin) family, originally identified as an inhibitory NK cell receptor (NKR) and negative regulator of NK activation, attributed to ITIM recruitment of SHP-1 phosphatase. Siglec 7 is expressed predominantly by natural killer cells (NK) and to a lesser extent by monocytes and granulocytes and, like Siglec-5 (CD170), has been shown to bind to sialylated ligands of targets through recognition of sialic acid in both the alpha-2,3- and alpha-2,6- glycosidic linkage.
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: frozen Optimal dilutions to be determined by the researcher.
IHC Positive Control Tissue
Frozen tonsil
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Hybridoma
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Monocyte-derived dendritic cells.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human Siglec 7.