Technical Data

S1013-68H
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mo
Accession #
NP_001128526
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-SIGIRR (Single Ig IL-1-Related Receptor, Single Ig IL-1R-Related Molecule, Single Immunoglobulin Domain-Containing IL1R-Related Protein, Toll/Interleukin-1 Receptor 8, TIR8, UNQ301/PRO342)
Single Immunoglobulin and Toll-interleukin 1 Receptor (TIR) Domain, HGNC:30575, MGC110992

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). TIR8, also know as single Ig IL-1 receptor, is a member of the IL-1:TLR superfamily (2). TIR8 acts as intracellular decoy to inhibit NF-kappaB activation by members of the IL-1:TLR family (3, 4).

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human or mouse kidney
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa334-358 of human TIR8.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Purity
Purified by Protein G affinity Chromatography.
Specificity
Recognizes human TIR8. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med. 187:2097-2101 (1998). 2. Thomassen E, Renshaw BR, and Sims JE. Cytokine 11: 389-399 (1999). 3. Polentarutti N, Penton R, Muzio G, et al. Eur Cytokine Network 14: 1-8 (2003). 4. Wald D, Qin J, Zhao Z, et al. Nat. Immunol. 4: 920-927 (2003).
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net