S1013-86ZB-ML650
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
MaxLight™650Isotype
IgG2aClone Number
10B870 (15-414)Grade
Affinity PurifiedApplications
FC IHCCrossreactivity
HuShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-SIRP alpha (Signal Regulatory Protein alpha, SIRPalpha, SIRPA, Signal Regulatory Protein alpha Type 1, Signal Regulatory Protein alpha 1, SIRP alpha 1, SIRPalpha1, Signal Regulatory Protein alpha Type 2, SIRP alpha 2, SIRPalpha2, SIRP alpha 3, Brain Immunoglobulin-like Molecule with Tyrosine-based Activation Motifs, Brain Ig-like Molecule with Tyrosine-based Activation Motifs, BIT, CD172a, CD172a Antigen, Inhibitory Receptor SHPS-1, Inhibitory Receptor SHPS1, Macrophage Fusion Receptor, MFR, MYD-1, Myd-1 Antigen, MYD1, Myd1 Antigen, p84, Protein Tyrosine Phosphatase Non-receptor Type Substrate 1, PTPNS1, SHP Substrate 1, SHPS1, SHPS-1, Tyrosine Phosphatase SHP Substrate 1, Tyrosine Protein Phosphatase Non-receptor Type Substrate 1, Tyrosine Protein Phosphatase Non-receptor Type Substrate) (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
SIRP alpha is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and hematopoietic stem cells. SIRP alpha acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:100-1:200. 10ul labels 10e6 cells in 100ul Immunohistochemistry (Frozen): 1:100 Optimal dilutions to be determined by the researcher.
Hybridoma
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Negative Control
I1904-83P1: IgG2a Murine Negative Control
Positive Control: Human tonsil
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Monocyte-derived dendritic cells.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human SIRP alpha.