Mouse Anti-SIRP, gamma (Signal Regulatory Protein gamma, SIRPG, CD172 gamma, CD172g, CD172g Antigen, Signal Regulatory Protein beta 2, SIRP beta 2, SIRPbeta2, SIRP-beta-2, SIRP B2, SIRPB2, SIRP-B2) (FITC)
SIRP gamma, a 55kD signal regulatory protein which is a member of the immunoglobulin gene superfamily. SIRP gamma, also known as CD172g, is expressed on most T lymphocytes and a subset of B-cells but is absent on myeloid cells. CD172g has a truncated cytoplasmic tail, which is similar to SIRP beta, but unlike SIRP beta CD172g does not require DAP12 for expression at the cell surface. CD172g, like SIRP alpha, binds to CD47 inducing apoptosis. It is also involved in the negative regulation of receptor tyrosine kinase-coupled signalling.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant SIRP gamma CD4.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human SIRP gamma.
