Technical Data

S1013-87KA-ML650
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
MaxLight™650
Isotype
IgG1
Clone Number
OX119
Grade
Affinity Purified
Applications
FC IP
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Mouse Anti-SIRP, gamma (Signal Regulatory Protein gamma, SIRPG, CD172 gamma, CD172g, CD172g Antigen, Signal Regulatory Protein beta 2, SIRP beta 2, SIRPbeta2, SIRP-beta-2, SIRP B2, SIRPB2, SIRP-B2) (MaxLight 650)

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Applications
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:50-1:200; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Hybridoma
NS-1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified recombinant SIRPgamma.CD4.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human SIRP gamma (CD172g).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Brooke, G. et al. (2004) Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family. J Immunol.173:2562-70.
USBio References
No references available
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