Technical Data

S1013-87Q
Clone Type
Monoclonal
Host
Mouse
Source
Rat
Isotype
IgG2a
Clone Number
OX-41
Grade
Affinity Purified
Applications
FC
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-SIRP, alpha1 (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a)
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry (frozen and paraffin). Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:100-1:200 Use 10ul to label 10e6 cells in 100ul Immunohistochemistry: Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Optimal dilution determined by the researcher
Recommended Secondary Reagents
IgG Goat anti mouse IgG:FITC (rat adsorbed) IgG Goat anti mouse IgG:RPE (rat adsorbed)
Recommended Secondary Reagents
Goat anti mouse IgG:HRP (rat adsorbed)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Rat peritoneal macrophages
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes rat CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. Binds to a different epitope on SIRP than ED9. Does not block the interaction of CD172a-CD47.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Robinson, A.P. et al. (1986). Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3. Immunology 57: 239-247. 2. Barclay, A.N. (1981). The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology 42: 593-600. 3. Adams, S. et al. (1998). Signal-regulatory protein is selectively expressed by myeloid and neuronal cells. J. Immunol. 161: 1853-1859.
USBio References
No references available
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