Mouse Anti-SIRP, alpha1 (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a)
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry (frozen and paraffin). Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:100-1:200 Use 10ul to label 10e6 cells in 100ul Immunohistochemistry: Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Optimal dilution determined by the researcher
Recommended Secondary Reagents
IgG Goat anti mouse IgG:FITC (rat adsorbed) IgG Goat anti mouse IgG:RPE (rat adsorbed)
Recommended Secondary Reagents
Goat anti mouse IgG:HRP (rat adsorbed)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Rat peritoneal macrophages
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes rat CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. Binds to a different epitope on SIRP than ED9. Does not block the interaction of CD172a-CD47.