Mouse Anti-SIRT1 (NAD-dependent Deacetylase Sirtuin-1, hSIRT1, SIR2-like Protein 1,SIR2L1 Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 1 (S. cerevisiae), hSIR2, SIR2L1) (Azide Free) (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. [provided by RefSeq]
Applications
Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1:500-1:1000 Immunofluorescence: 10ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Partial recombinant protein corresponding to a portion of amino acids within aa456-555, from human SIRT1 with GST tag. GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SIRT1 at ~81kD.
