Technical Data

S1014-16D
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_008861; P63208; Q71U00; Q6PEC4; Q9WTX5; Q4R5B9; Q5ZKF5; Q3ZCF3
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-SKP1, ID (S-phase Kinase-associated Protein 1, Cyclin A/CDK2-associated Protein p19, p19A, p19skp1, RNA Polymerase II Elongation Factor-like Protein, Organ Of Corti Protein 2, OCP-II Protein, OCP-2, Transcription Elongation Factor B, SIII, SKP1A, EMC19, OCP2, SKP1, TCEB1L)

SKP1A is an F-box protein which functions as a substrate recognition component of the SCF ubiquitin ligase complex. It binds to cyclin F, S-phase kinase-associated protein 2, and other regulatory proteins involved in ubiquitin proteolysis through an F-box motif. SKP1A also collaborates with a network of proteins to control beta-catenin levels and affects the activity level of beta-catenin dependent TCF transcription factors. Studies have also characterized the protein as an RNA polymerase II elongation factor.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 58-87 in the central region of human SKP1, UniProt Accession #NP_008861; P63208. Species sequence homology: bovine, chicken, monkey mouse, rat, Xenopus
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human SKP1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Wu, G., et al., Mol. Cell 11(6):1445-1456 (2003). 2.Piva, R., et al., Mol. Cell. Biol. 22(23):8375-8387 (2002). 3.Matsuzawa, S.I., et al., Mol. Cell 7(5):915-926 (2001). 4.Schulman, B.A., et al., Nature 408(6810):381-386 (2000). 5.Winston, J.T., et al., Genes Dev. 13(3):270-283 (1999).
USBio References
No references available
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