Sheep Anti-SMAD9 (Mothers Against Decapentaplegic Homolog 9, MAD Homolog 9, Mothers Against DPP Homolog 9, Madh6, SMAD Family Member 9, SMAD 9, Smad9, MADH6, MADH9)
SMAD9 is a 55-60kD R-Group member of the dwarfin/SMAD family of proteins. It is expressed by BMP-responsive cells, and serves to transmit information from the BMP receptor (BMPR) to the nucleus. In particular, following BMPR activation, SMAD9 is phosphorylated by the BMPR on its C-terminus, promoting heterodimerization with SMAD4 and its translocation into the nucleus. Here, SMAD9 binds to DNA and participates in gene activation. Human SMAD9 is 467aa in length. It contains a DNA-binding domain termed MH1 aa16-140 plus a receptor/transcription factor binding domain termed MH2 aa273-467. The C-terminus contains two phosphorylation sites at aa465 and aa467. There is one splice form that shows a deletion of aa224-260. Over aa158-223, human and mouse SMAD9 share 82% identy in aa sequence.
Applications
Suitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 5-15ug/ml Immunocytochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant corresponding to aa158-223 from human SMAD9 expressed in E.coli.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SMAD9.
