Rabbit Anti-SMARCA4 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily a, Member 4, BAF190, BRG1, SNF2-BETA, SNF2L4, SNF2LB, hSNF2b)
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.
Applications
Suitable for use in Western Blot, Immunoprecipitation, ChIP, Immunohistochemistry and Proximity Ligation Assay. Other applications not tested.
Recommended Dilutions
Immunoprecipitation: 2-5ug/mg lysate Western Blot: 1:2000-1:10,000 Proximity Ligation Assay: 1:200-1:2000 Immunohistochemistry: 1:250-1:2000 Immunohistochemistry (paraffin): 1:10-1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to a region between residues 75-125 of human SMARCA4 using the numbering given in entry NP_003063.2 (GeneID 6597).
Form
Supplied as a liquid in Tris-buffered saline, pH 7.2, 0.1% BSA, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SMARCA4. Species Crossreactivity: mouse
