Rabbit Anti-SMURF2, NT (E3 Ubiquitin-protein Ligase SMURF2, Smad Ubiquitination Regulatory Factor 2, Ubiquitin-protein Ligase SMURF2, Smad-specific E3 Ubiquitin Ligase 2, hSMURF2)
SMURF2, a 748aa ubiquitin E3 ligase that is 83% identical to SMURF1, codes for a C2-WW-HECT domain ubiquitin ligase that associates constitutively with SMAD7. Binding to SMAD7 induces export of SMURF2 and recruitment to the activated transforming growth factor-beta receptor (TGFBR), where it causes receptor and SMAD7 degradation. A strong interaction of second and third SMURF2 WW domains has been identified with SMAD1, SMAD2, and SMAD3, but not SMAD4. Western blot analysis showed that SMURF2 selectively downregulates the transcription of SMAD2 and SMAD1, but not SMAD3. The nuclear SMURF2/phosphorylated SMAD2 interaction is requires TGFB1.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 6-36 in the N-terminal region of human SMURF2.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human SMURF2. Species Crossreactivity: mouse.
