Mouse Anti-SLURP1, Secreted Ly-6/uPAR-related Protein 1
Secreted Ly-6/uPAR-related protein 1, SLURP1, is a 9kD secreted member of the Ly-6/uPAR protein superfamily. Human SLURP1 is synthesized as a 103aa precursor that contains a 21aa signal sequence and an 82aa mature chain. Like other members of the Ly-6/uPAR family, SLURP1 contains a Ly-6/uPAR domain (aa24-73) and ten cysteine residues that make up five disulfide bonds. Unlike other family members, however, SLURP1 does not contain a GPI anchor. Mature human SLURP1 shares 67% and 68% aa identity with mouse and rat SLURP1, respectively. The protein forms a homodimer and is expressed in skin, eye, whole lung, trachea, esophagus, and stomach. In humans, SLURP1 is involved in maintaining the integrity of the kerotinocyte layers of the skin and has demonstrated antitumor activity.
Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 8-25ug/ml with immersion fixed paraffin-embedded sections of human lung and human placenta Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Ala22-Leu103 of human SLURP1 expressed in E. coli.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human SLURP1. Does not react with recombinant mouse SLURP1
