Goat Anti-SNX1 (Sorting Nexin 1, SNX-1, Sorting Nexin 1A, SNX1A, HsT17379, MGC8664)
HsT17379, MGC8664, SNX1A, Sorting nexin-1, Vps5
This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. This protein also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members. This gene results in three transcript variants encoding distinct isoforms.
Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Peptide ELISA Titer: 1:64,000 Western Blot: 1-3ug/ml ~75kD band observed in Human lysates of cell line HeLa (calculated MW of 59.1kD according to NP_003090.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-TNSSKPQPTYEELE, from the internal region of human SNX1 (NP_003090.2; NP_690039.1).
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes human SNX1. Species sequence homology: mouse, rat, canine, bovine. This antibody is expected to recognize isoform a and c (NP_003090.2; NP_690039.1).
