Rabbit Anti-SOCS 1(Suppressor of Cytokine Signalling 1, Janus kinase (JAK) binding protein, STAT-induced STAT inhibitor, SSI-1) (MaxLight 650)
MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
SOCS-1, also called Janus kinase (JAK) binding protein (JAB), or STAT-induced STAT inhibitor (SSI-1), is an SH2-domaincontaining protein. It was shown to interact with all four Jak kinases (Jak1, Jak2, Jak3 and Tyk2) and is induced by and inhibits the signaling action of IL-3, IL-4, IL-6, growth hormone, LIF, prolactin, interferon gamma (IFN-g), and erythropoietin. It plays an important role in the negative regulation of IFN-g signaling and in the differentiation of T-cells. SOCS-1 mRNA is highly expressed in the thymus, to a much lower level in spleen, and not expressed in most other tissues. Biochemical characterization as well as gene disruption studies indicate that SOCS-1 is an important negative regulator of the JAK-STAT signal pathway.
Applications
Suitable for use in Western Blot. Other applications have not been tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Positive Controls
Mouse thymus cell lysates and HepG2 human hepatocellular carcinoma lysate.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
A synthetic peptide derived from the C-terminal region of human, mouse or rat SOCS-1 protein
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes the human SOCS-1 protein at ~30kD. Species Crossreactivity: mouse Species Sequence Homology: rat
