Goat Anti-SLC12A2 (Solute Carrier Family 12 Member 2, Basolateral Na-K-Cl Symporter, Bumetanide-sensitive Sodium-(potassium)-chloride Cotransporter 1, NKCC1)
Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
Applications
Suitable for use in ELISA, Flow Cytometry, Immunofluorescenc and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Peptide ELISA: : 1:64,000 Immunohistochemistry (Paraffin): 3.75ug/ml Flow Cytometry: 10ug/ml Immunofluorescence (IC): 10ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-NALQHSIRLSGVEDH of the internal region of human SLC12A2 Species sequence homology: Bovine, canine, mouse and rat.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SLC12A2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Pawelczyk M, Van Laer L, Fransen E, Rajkowska E, Konings A, Carlsson PI, Borg E, Van Camp G, Sliwinska-Kowalska M, Analysis of gene polymorphisms associated with K ion circulation in the inner ear of patients susceptible and resistant to noise-induced hearing loss. Annals of human genetics 2009 Jul 73(Pt 4):411-21.
USBio References
No references available
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