Rabbit Anti-Somatostatin 28 (Somatostatin-28, Growth Hormone Release Inhibiting Factor, Somatostatin Precursor, SMST, SST)
Somatostatin (also known as growth hormone inhibiting hormone (GHIH) or somatotropin release-inhibiting factor (SRIF) is widely distributed throughout the body. It has two active forms produced by alternative cleavage of a single preproprotein: one of 14 amino acids, the other of 28 amino acids. Somatostatin is an important regulator of endocrine and nervous system function. It exerts its biologic actions by binding to specific high affinity receptors on the cell surface. Somatostatin has also been shown in sympathetic nerves, mucosal cells, myenteric nerves of the gastrointestinal tract, salivary glands and in some parafollicular cells of the thyroid.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1:3200 (0.313ug/ml) Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to Ser-Ala-Asn-Ser-Asn-Pro-Ala- Met-Ala-Pro-Arg-Glu-Arg-Lys-Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys- Thr-Phe-Thr-Ser-Cys (Disulfide Bridge: Cys17-Cys28) of human Somatostatin-28.
Form
Supplied as a lyophilized powder. Reconstitute with PBS.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human Somatostatin-28. Species crossreactivity: rat, mouse, porcine.