Technical Data

S5364-44F
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_008873.100
Gene ID
6665
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-SOX15 (Protein SOX-15, Protein SOX-12, Protein SOX-20, SOX12, SOX20, SOX26, SOX27)

This member of the SOX (SRY-related HMG-box) family of transcription factors is involved in the regulation of embryonic development and in the determination of the cell fate. It may act as a transcriptional regulator after forming a protein complex with other proteins. It binds to the 5'-AACAAT-3' sequence and contains 1 HMG box domain.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:16,000 Western Blot: 0.1-0.3ug/ml, observed in Human Skeletal Muscle lysates on ~24kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to CSLPQSDPRLQGE, from human SOX15, at the internal region of the protein (NP_008873.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SOX15.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Meeson AP, Shi X, Alexander MS, Williams RS, Allen RE, Jiang N, Adham IM, Goetsch SC, Hammer RE, Garry DJ. Sox15 and Fhl3 transcriptionally coactivate Foxk1 and regulate myogenic progenitor cells. EMBO J. 2007 Apr 4;26(7):1902-12. Epub 2007 Mar 15.
USBio References
No references available
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