Goat Anti-SOX17 (SRY-Box Containing Gene, Sex Determining Region Y-Box 17, SRY, Transcription Factor SOX17) (NL®557)
SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development.
Applications
Suitable for use in Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Immunocytochemistry: 1:10: Tested using definitive endoderm differentiated BG01V cells. Cells were fixed in PBS containing 4% paraformaldehyde, and blocked with PBS containing 10% normal donkey serum, 0.1% Triton® X-100, and 1% BSA. After blocking, cells were incubated with S5364-45A (1:10) in blocking buffer for 3 hours in the dark. Between each step, cells were washed with PBS containing BSA. If a staining volume of 20 tests can be done in a staining volume of 250ul; 100 tests can be done in a staining volume of 50ul. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. NorthernLights 557 conjugates are sensitive to light.
Immunogen
Recombinant protein corresponding to aa177-414 of human SOX17 expressed in E. coli. (Q9H612)
Form
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with NorthernLights ®557.
Purity
Purified by Protein G affinity chromatography
Specificity
Recognizes human SOX17. ≤1% cross-reactivity with recombinant human (rh) SOX2, rhSOX6, and rhSOX15.
