Mouse Anti-Spectrin, beta, Erythrocytic (EL3, HS2, Erythroid beta-Spectrin, SPTB1, Spectrin beta Chain Erythrocyte, SPTB, Beta-I Spectrin, HSPTB1, SPH2)
Erythroid beta-Spectrin
Erythroid spectrins, some other proteins of erythroid cytoskeleton, and the transmembrane protein band 3 are highly specific for erythrocytes and their progenitors. They are more reliable markers for erythroid differentiation than Glycophorin A, the commonly used marker for erythroid differentiation, because Glycophorin A is expressed also in many cell lines otherwise exhibiting mainly megakaryotic charasteristics.
Applications
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Ghost proteins of human red blood cells
Form
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide.
Specificity
Recognizes the 220kD human erythroid b-spectrin. Present in the membranes of human skeletal muscle cells.
