S5401-36
Clone Type
PolyclonalHost
GoatSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
ECrossreactivity
HuAccession #
NP_078808.3; NP_001020607.1Shipping Temp
Blue IceStorage Temp
-20°CGoat Anti-SPAG16 (Sperm Associated Antigen 16 aa139-152)
SPAG16, WDR29, WD repeat domain 29, sperm-associated WD repeat protein, sperm associated antigen 16, PF20, MGC87036, FLJ37717, FLJ22724, DKFZp666P1710
Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively (Zhang et al., 2007 [PubMed 17699735]).
Applications
Suitable for use in ELISA. Other applications have not been tested.
Recommended Dilutions
Peptide ELISA Titer: 1:16,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-KGVTELRTVGNVPD, from the Internal region of human SPAG16 (NP_078808.3; NP_001020607.1).|
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes SPAG16. Species sequence homology: human, canine, bovine.|This antibody is expected to recognize isoform 1 and 2 (NP_078808.3; NP_001020607.1).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Zhang Z, Zariwala MA, Mahadevan MM, Caballero-Campo P, Shen X, Escudier E, Duriez B, Bridoux AM, Leigh M, Gerton GL, Kennedy M, Amselem S, Knowles MR, Strauss JF,A heterozygous mutation disrupting the SPAG16 gene results in biochemical instability of central apparatus components of the human sperm axoneme.Biology of reproduction 2007 Nov 77 (5): 864-71.USBio References
No references available